Anti-Tlr5 Antibody Picoband™

Product Name

Anti-Tlr5 Antibody Picoband™

View all TLR5 Antibodies

SKU/Catalog Number

A00462-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tlr5 Antibody Picoband™ catalog # A00462-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Tlr5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00462-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Tlr5 recombinant protein (Position: Q81-D766).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00462-2 is reactive to TLR5 in Mouse, Rat

Applications

A00462-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

97.834kDa

Background of TLR5

Toll-like receptor 5, also known as TLR5, is a protein which in humans is encoded by the TLR5 gene. Predicted to enable interleukin-1 receptor binding activity and signaling receptor activity. Predicted to be involved in several processes, including positive regulation of interleukin-8 production; positive regulation of nitric oxide biosynthetic process; and positive regulation of toll-like receptor signaling pathway. Predicted to act upstream of or within innate immune response. Predicted to be located in membrane. Predicted to be integral component of plasma membrane. Is expressed in brain; olfactory epithelium; spinal cord; and trunk unsegmented mesenchyme. Human ortholog(s) of this gene implicated in Legionnaires' disease; cystic fibrosis; cystitis; melioidosis; and systemic lupus erythematosus. Orthologous to human TLR5 (toll like receptor 5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Tlr5 using anti-Tlr5 antibody (A00462-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr5 antigen affinity purified polyclonal antibody (Catalog # A00462-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr5 at approximately 100 kDa. The expected band size for Tlr5 is at 98 kDa.

Gene/Protein Information For TLR5 (Source: Uniprot.org, NCBI)

Alternative Names

MGC126430; MGC126431; SLEB1FLJ10052; TIL-3; TIL3Toll/interleukin-1 receptor-like protein 3; TLR5; toll-like receptor 5 TLR5 MELIOS, SLE1, SLEB1, TIL3 toll like receptor 5 toll-like receptor 5|systemic lupus erythematosus susceptibility 1|toll/interleukin-1 receptor-like protein 3

For more info on TLR5, check out the TLR5 Infographic

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In this infographic, you will see the following information for TLR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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